The fluid should immediately be placed into proper collecting tubes. Complete analysis can usually be performed with as little as 10-15 cc of fluid (7). However, if only a few ml of fluid are obtained, cultures and microscopy should receive priority. All tubes should be filled at least half way.
- RED TOP (no additive): Sent for chemistries such as protein and glucose. Of note, serum specimens for reference should also be sent at this time.
- PUPRLE TOP (EDTA prevents clotting): This tube is used for microscopy, cell count and differentials
- GREEN TOP (Sodium heparin prevents clotting and does not crystallize (unlike EDTA)): This tube should be sent for crystal examination.
The remaining fluid (ideally at least 5 cc) should be placed in appropriate culture media.
A clean dry sterile dressing or adhesive bandaging should be applied to the wound. The patient may take NSAIDs for pain control, if not contraindicated. The patient should be instructed to look for signs of local infection including erythema, warmth, or increased swelling, or systemic signs such as fevers and chills. A significant increase in pain and swelling can also occur if a large hematoma develops. Patients should report these symptoms to their doctors. The joint should be rested from strenuous activity as well (15).
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